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KMID : 0378019790220120105
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New Medical Journal
1979 Volume.22 No. 12 p.105 ~ p.109
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Changes of Protein, Reduced and Oxidized Glutathione Levels of the Rat Kidney Following the Repeated Administration of Ethanol
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Abstract
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In the present study, an effort was directed to elucidate the possible changes of protein and both reduced (GSH) and oxidized (GSSG) glutathione levels of the rat kidney following the repeated administration of alcohol.
A daily dose of 1.0ml of 33% alcohol per 100 gm BW was orally ingested to albino rats repeatedly for 40 days, and the experiment was performed at 5 days interval from the 20th day of alcohol ingestion.
The rat was sacrificed by a heavy blow to the head, and the kidneys were excised after opening the abdominal cavity. The cortex of the kidney was taken for the sample, and the protein level was determined by the Lowry, et al method, GSH by the ElIman´s method and GSSG by the electrolytic reduction method described by Dohan and Woodward. The control was set in which the same amount of water in place of alcohol was ingested, and the rest of the procedure was identical with the experimental group.
The results were compared with,the normal values, and summarized as follows. The protein level of the normal rat kidney was 169.55.98mg/gm, while the alcohol group showed a significantly increased protein level throughout the entire experiment with the highest level on 35th and 40th day of alcohol ingestion. The GSH level of the rat kidney in the alcohol group was generally. decreased comparing with the normal with the significant decrease on 20th and 30th day of the experiment. The GSSG level of the rat kidney in the alcohol group showed a generally increased tendency throughout the experiment comparing with the normal value of 6.160.50pmol/gm.
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